RNAseq and Methylseq



Biotechvana is pleased to offer data analysis services for comparative studies to test differential expression of mRNA and non-coding RNA (both long and small) between different RNAseq samples and conditions. We also offer analytical services for epigenetic studies to test differential methylation of gene features and regulatory elements(CpG islands, promoters, introns, exons) between Methylseq samples o similar (e.g. inmmuno-precipitation). Although each study needs to be personalized depending on your experiment design and the species to be resequenced, below we summarize the usual workflow followed by us in these types of studies.

RNAseq Studies


For mRNA and Long ncRNA

1) Preprocessing of raw data 2) Mapping on reference 3) Quantification and normalization 4) Testing Differential Expression 5) Downstream analyses
For Small and micro RNAs

1) Preprocessing of raw data 2) Mapping on reference 3) Quantification and normalization 4) Testing Differential Expression 5) Downstream analyses

Methylseq and other epigenetic studies


1) Preprocessing of raw data 2) Mapping on reference 3) Testing differential methylation 4) Downstream analyses

If you are interested in getting more information about our services for analysis of RNAseq or Methylseq data, please contact us at biotechvana(at)biotechvana.com and we will be glad to pass you on a quote or organize a personalized meeting with you in order to discuss and evaluate your experiment.

Related publications



  • Kanoni S (and 29 co-authors including Llorens C). 2021. Nutrigenetic Interactions Might Modulate the Antioxidant and Anti-Inflammatory Status in Mastiha-Supplemented Patients With NAFLD. Frontiers in Immunology, 12, 1688 doi: https://doi.org/10.3389/fimmu.2021.683028
  • Pérez-Sánchez R, Carnero-Morán Á, Soriano B, Llorens C, Oleaga A. 2021. RNA-seq analysis and gene expression dynamics in the salivary glands of the argasid tick Ornithodoros erraticus along the trophogonic cycle. Parasites Vectors 14, 170. https://doi.org/10.1186/s13071-021-04671-z
  • Herreros-Pomares A, Llorens C, Soriano B, Bagan L, Moreno A, Calabuig-Fariñas S, Jantus-Lewintre E, Bagan J. 2021. Differentially methylated genes in proliferative verrucous leukoplakia reveal potential malignant biomarkers for oral squamous cell carcinoma, Oral Oncology, Volume 116,105191https://doi.org/10.1016/j.oraloncology.2021.105191
  • Amerikanou C and 32 coauthors (including Soriano B, Llorens C and Roig FJ). 2021.Effect of Mastiha supplementation on NAFLD: The MAST4HEALTH Randomised, Controlled Trial. Mol Nutr Food Res. Feb 24:e2001178. Epub ahead of print. PMID: 33629536.https://doi.org/10.1002/mnfr.202001178
  • Oleaga A, Soriano B, Llorens C, Pérez-Sánchez R. 2021. Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle. PLOS Neglected Tropical Diseases 15(2): e0009105.https://doi.org/10.1371/journal.pntd.0009105
  • Llorens C, Soriano B, Trilla-Fuertes L, Bagan L, Ramos-Ruis R, Gamez-Pozo A, Peña C, Bagan JV. 2020. Immune expression profile identification in a group of proliferative verrucous leukoplakia patients: a pre-cancer niche for oral squamous cell carcinoma development. Clin Oral Invest. https://doi.org/10.1007/s00784-020-03575-z
  • Hovhannisyan H, Hafez A, Llorens C, Gabaldon T. 2020. CROSSMAPPER: estimating cross-mapping rates and optimizing experimental design in multi-species sequencing studies. Bioinformatics. 36(3): 925-927 https://doi.org/10.1093/bioinformatics/btz626
  • Díaz R, Sandoval P, Rodrigues-Diez RR, del Peso G, Jiménez-Heffernan JA, Ramos-Ruíz R, Llorens C, Laham G, Alvarez-Quiroga M, López-Cabrera M, Ruiz-Ortega M, Bajo M.A, Selgas R. 2020. Increased miR-7641 Levels in Peritoneal Hyalinizing Vasculopathy in Long-Term Peritoneal Dialysis Patients. Int. J. Mol. Sci. 21 5824. https://doi.org/10.3390/ijms21165824
  • Pérez-Sánchez J, Naya-Català F, Soriano B, Piazzon MA, Hafez A, Gabaldón T, Llorens C, Sitjà-Bobadilla A, Calduch-Giner J. 2019. Genome Sequencing and Transcriptome Analysis Reveal Recent Species-specific Gene Duplications in the Plastic Gilthead Sea Bream. Front. Mar. Sci. 20 https://doi.org/10.3389/fmars.2019.00760
  • OPATHY Consortium (including Hafez A, and Llorens C), Gabaldon T. 2019. Recent trends in molecular diagnostics of yeast infections: from PCR to NGS. FEMS microbiology reviews.43(5); 517–547 https://doi.org/10.1093/femsre/fuz015
  • Rodríguez-García E, Olagüe C, Ríus-Rocabert S, Ferrero R, Llorens C, Larrea E, Fortes P, Prieto J, González-Aseguinolaza G, Nistal-Villan E.2018 TMEM173 Alternative Spliced Isoforms Modulate Viral Replication through the STING Pathway. ImmunoHorizons, 2,11 pp 363-376. http://www.immunohorizons.org/content/2/11/363
  • Llorens C, Arcos SC, Robertson L, Ramos R, Futami R, Soriano B, Ciordia S, Careche M, González-Muñoz M, Jiménez-Ruiz Y, Carballeda-Sangiao N, Moneo I, Albar JP, Blaxter M and Navas A. 2018.Functional insights into the infective larval stage of Anisakis simplex s.s., Anisakis pegreffii and their hybrids based on gene expression patterns. BMC Genomics 19:592. https://doi.org/10.1186/s12864-018-4970-9
  • Herrera M, Llorens C, Rodríguez M, Herrera A, Ramos R, Gil B, Candia A, Larriba MJ, Garre P, Earl J, Rodríguez-Garrote M, Caldés T, Bonilla F, Carrato A, García-Barberán V and Peña C. 2018. Differential distribution and enrichment of non-coding RNAs in exosomes from normal and Cancer-associated fibroblasts in colorectal cancer. Molecular Cancer, 17:114 http://doi.org/10.1186/s12943-018-0863-4
  • Saus E, Willis JR, Pryszcz LP, Hafez A, Llorens C, Himmelbauer H, Gabaldon T. 2018. nextPARS: Parallel probing of RNA structures in Illumina. Published in Advance January 22, 2018, RNA doi: 10.1261/rna.063073.117



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    Hipra Scientific S.L.U, Polypeptide Therapeutic Solutions S.L., Biotechvana S.L. and Nostrum Biodiscovery constitute the consortium of enterprises participating in the project "Research of a new vaccine for a human respiratory disease", granted by the CDTI (Center for Industrial Technological Development), and supported by the Ministry of Science and Innovation and financed by the European Union – NextGenerationEU. The main objective of this project is to design a safe immunogenic and effective vaccine against the respiratory syncytial virus.

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